Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

ASF1B ELISA Kit

ASF1B Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730407
  • Target Alle ASF1B Produkte
    ASF1B (ASF1 Anti-Silencing Function 1 Homolog B (ASF1B))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human ASF1B ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human ASF1B.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle ASF1B Produkte
    ASF1B (ASF1 Anti-Silencing Function 1 Homolog B (ASF1B))
    Andere Bezeichnung
    ASF1B (ASF1B Produkte)
    Synonyme
    CIA-II ELISA Kit, asf1-b ELISA Kit, asf1a ELISA Kit, asf1a-b ELISA Kit, asf1ab ELISA Kit, cia-ii ELISA Kit, 1700003K02Rik ELISA Kit, AA409591 ELISA Kit, asf1b ELISA Kit, fc68a10 ELISA Kit, wu:fc68a10 ELISA Kit, zgc:77178 ELISA Kit, ASF1B ELISA Kit, F16F17.110 ELISA Kit, F16F17_110 ELISA Kit, SGA01 ELISA Kit, SGA1 ELISA Kit, anti- silencing function 1b ELISA Kit, asf1 ELISA Kit, MGC89345 ELISA Kit, asb1bb ELISA Kit, wu:fb20g03 ELISA Kit, zgc:76977 ELISA Kit, anti-silencing function 1B histone chaperone ELISA Kit, anti-silencing function 1B histone chaperone L homeolog ELISA Kit, anti-silencing function 1Ba histone chaperone ELISA Kit, anti- silencing function 1b ELISA Kit, histone chaperone ASF1B ELISA Kit, anti-silencing function 1Bb histone chaperone ELISA Kit, ASF1B ELISA Kit, asf1b.L ELISA Kit, Asf1b ELISA Kit, asf1ba ELISA Kit, LOC9304878 ELISA Kit, asf1b ELISA Kit, asf1bb ELISA Kit
    UniProt
    Q9NVP2
    Pathways
    Regulation of Cell Size
Sie sind hier:
Kundenservice