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BAFF ELISA Kit
TNFSF13B
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730178
Produktdetails BAFF ELISA Kit
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Target
Alle BAFF (TNFSF13B) ELISA Kits anzeigen
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Reaktivität
Alle Reaktivitäten für BAFF ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse BAFF/BLyS/TNFSF13B ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse BAFF.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product TNFSF13B ELISA Kit
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu BAFF
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Target
Alle BAFF (TNFSF13B) ELISA Kits anzeigen
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Andere Bezeichnung
BAFF (BLyS/TNFSF13B) (TNFSF13B Produkte )
Synonyme
BAFF ELISA Kit, BLYS ELISA Kit, CD257 ELISA Kit, DTL ELISA Kit, TALL-1 ELISA Kit, TALL1 ELISA Kit, THANK ELISA Kit, TNFSF20 ELISA Kit, ZTNF4 ELISA Kit, BAFF-R ELISA Kit, BAFFR ELISA Kit, BROMIX ELISA Kit, CD268 ELISA Kit, CVID4 ELISA Kit, prolixin ELISA Kit, TNFSF13B ELISA Kit, RGD1561519 ELISA Kit, RGD1560810 ELISA Kit, BLyS ELISA Kit, D8Ertd387e ELISA Kit, zTNF4 ELISA Kit, 2010006P15Rik ELISA Kit, Baffr ELISA Kit, Bcmd ELISA Kit, Bcmd-1 ELISA Kit, Bcmd1 ELISA Kit, Lvis22 ELISA Kit, zgc:172115 ELISA Kit, TNF superfamily member 13b ELISA Kit, TNF receptor superfamily member 13C ELISA Kit, tumor necrosis factor (ligand) superfamily, member 13b ELISA Kit, tumor necrosis factor receptor superfamily, member 13c ELISA Kit, tumor necrosis factor superfamily member 13b ELISA Kit, TNFSF13B ELISA Kit, TNFRSF13C ELISA Kit, Tnfsf13b ELISA Kit, Tnfrsf13c ELISA Kit, tnfsf13b ELISA Kit
UniProt
Q9WU72
Pathways
NF-kappaB Signalweg , Production of Molecular Mediator of Immune Response
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