S100A8 ELISA Kit
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- Target Alle S100A8 ELISA Kits anzeigen
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.65 ng/mL - 150 ng/mL
- Untere Nachweisgrenze
- 0.65 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Mouse S100A8/S100A9 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes Mouse S100A8.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product S100A8 ELISA Kit
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Aufbereitung der Reagenzien
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Recommended Dilution for serum and plasma samples2 fold
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle S100A8 ELISA Kits anzeigen
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- Andere Bezeichnung
- S100A8 (S100A9 (S100A8 Produkte)
- Synonyme
- 60B8AG ELISA Kit, CAGA ELISA Kit, CFAG ELISA Kit, CGLA ELISA Kit, CP-10 ELISA Kit, L1Ag ELISA Kit, MA387 ELISA Kit, MIF ELISA Kit, MRP8 ELISA Kit, NIF ELISA Kit, P8 ELISA Kit, Mrp8 ELISA Kit, S100A8 ELISA Kit, 60B8Ag ELISA Kit, AI323541 ELISA Kit, B8Ag ELISA Kit, CFAg ELISA Kit, Caga ELISA Kit, p8 ELISA Kit, MRP-8 ELISA Kit, S100 calcium binding protein A8 ELISA Kit, S100 calcium binding protein A8 (calgranulin A) ELISA Kit, S100A8 ELISA Kit, S100a8 ELISA Kit
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine
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