NBL1 ELISA Kit

Produktnummer ABIN6720249

Produktdetails NBL1 ELISA Kit

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: D1S1733E, DAN, DAN domain family member 1, DAND 1, DAND1, Differential screening selected gene aberrant in neuroblastoma, MGC8972, NB, NBL 1, Neuroblastoma candidate region suppression of tumorigenicity 1, Neuroblastoma suppression of tumorigenicity 1, Neuroblastoma suppressor of tumorigenicity 1, NO 3, NO3, Zinc finger protein DAN

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Gene Name: NBL1

Production: Natural and recombinant mouse DAN. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A17-D178

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse DAN standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu NBL1

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Andere Bezeichnung: DAN - NBL1 (NBL1 Produkte)

Synonyme: D1S1733E ELISA Kit, DAN ELISA Kit, DAND1 ELISA Kit, NB ELISA Kit, NO3 ELISA Kit, D4H1S1733E ELISA Kit, Dana ELISA Kit, dand1 ELISA Kit, nbl1-a ELISA Kit, no3 ELISA Kit, 4.1N ELISA Kit, Epb41l1 ELISA Kit, NBL1 ELISA Kit, mKIAA0338 ELISA Kit, C1orf151-NBL1 ELISA Kit, dan ELISA Kit, zgc:77251 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist ELISA Kit, neuroblastoma, suppression of tumorigenicity 1 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist L homeolog ELISA Kit, neuroblastoma suppressor of tumorigenicity 1 ELISA Kit, erythrocyte membrane protein band 4.1 like 1 ELISA Kit, MINOS1-NBL1 readthrough ELISA Kit, NBL1 ELISA Kit, Nbl1 ELISA Kit, nbl1.L ELISA Kit, LOC535280 ELISA Kit, Epb41l1 ELISA Kit, MINOS1-NBL1 ELISA Kit, nbl1 ELISA Kit

Gen-ID: 17965

NCBI Accession: NP_032701

UniProt: Q61477