CD21 ELISA Kit

Produktnummer ABIN6720009

Produktdetails CD21 ELISA Kit

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: C3DR, CD 21, CD21, CD21 antigen, Complement C3d receptor, Complement component (3d/Epstein Barr virus) receptor 2, Complement component receptor 2, Complement receptor type 2, Cr 2, CR, Cr2, CR2_HUMAN, CVID7, EBV receptor, EBV-R, Epstein Barr virus receptor, Epstein-Barr virus receptor, EVBR, SLEB9

Background: Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).

Gene Name: CR2

Production: Natural and recombinant human CD21. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: Ile21-71

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human CD21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD21 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Andere Bezeichnung: CD21 - CR2 (CR2 Produkte)

Synonyme: C3DR ELISA Kit, CD21 ELISA Kit, CR ELISA Kit, CVID7 ELISA Kit, SLEB9 ELISA Kit, Cd21 ELISA Kit, cd21 ELISA Kit, CR1 ELISA Kit, CR1-L ELISA Kit, CR2 ELISA Kit, complement C3d receptor 2 ELISA Kit, complement C3b/C4b receptor 1 (Knops blood group) ELISA Kit, CR2 ELISA Kit, Cr2 ELISA Kit, CR1 ELISA Kit

Gen-ID: 1380

NCBI Accession: NP_001006659

UniProt: P20023

Pathways: Komplementsystem