Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

EGFR IQ-ELISA Kit

EGFR Reaktivität: Hund qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN6384484
  • Target Alle EGFR IQ-ELISA Kits anzeigen
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Reaktivität
    • 35
    • 25
    • 23
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Hund
    Nachweismethode
    qPCR
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Canine Immunoquantitative (PCR-Based) EGFR ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Semi-Quantitative
    Produktmerkmale
    IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
    Bestandteile
    • EGFR Microplate (Item A): 96 well PCR plate coated with anti-Canine EGFR
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Canine EGFR
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for EGFR (Item F): 2 vials of a 4x concentrated solution of anti-Canine EGFR affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Benötigtes Material
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
    Featured
    Zu unserem meistverkauften EGFR ELISA Kit
    Top Product
    Discover our top product EGFR ELISA Kit
  • Applikationshinweise
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Kommentare

    The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

    Probenmenge
    25 µL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target Alle EGFR IQ-ELISA Kits anzeigen
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Andere Bezeichnung
    EGFR (EGFR Produkte)
    Synonyme
    C-erb IQ-ELISA Kit, CG10079 IQ-ELISA Kit, D-EGFR IQ-ELISA Kit, D-Egf IQ-ELISA Kit, DEGFR IQ-ELISA Kit, DER IQ-ELISA Kit, DER flb IQ-ELISA Kit, DER/EGFR IQ-ELISA Kit, DER/faint little ball IQ-ELISA Kit, DER/top IQ-ELISA Kit, DER/torpedo IQ-ELISA Kit, DER1 IQ-ELISA Kit, DEgfr IQ-ELISA Kit, Degfr IQ-ELISA Kit, Der IQ-ELISA Kit, DmHD-33 IQ-ELISA Kit, Dmel\\CG10079 IQ-ELISA Kit, EFG-R IQ-ELISA Kit, EGF-R IQ-ELISA Kit, EGFR IQ-ELISA Kit, EGFr IQ-ELISA Kit, EGfr IQ-ELISA Kit, EK2-6 IQ-ELISA Kit, Egf IQ-ELISA Kit, Egf-r IQ-ELISA Kit, EgfR IQ-ELISA Kit, El IQ-ELISA Kit, Elp IQ-ELISA Kit, Elp-1 IQ-ELISA Kit, Elp-B1 IQ-ELISA Kit, Elp-B1RB1 IQ-ELISA Kit, HD-33 IQ-ELISA Kit, TOP IQ-ELISA Kit, Torpedo/DER IQ-ELISA Kit, Torpedo/Egfr IQ-ELISA Kit, c-erbB IQ-ELISA Kit, d-egf-r IQ-ELISA Kit, dEGFR IQ-ELISA Kit, dEGFR1 IQ-ELISA Kit, dEgfr IQ-ELISA Kit, der IQ-ELISA Kit, egfr IQ-ELISA Kit, flb IQ-ELISA Kit, l(2)05351 IQ-ELISA Kit, l(2)09261 IQ-ELISA Kit, l(2)57DEFa IQ-ELISA Kit, l(2)57EFa IQ-ELISA Kit, l(2)57Ea IQ-ELISA Kit, mor1 IQ-ELISA Kit, top IQ-ELISA Kit, top/DER IQ-ELISA Kit, top/flb IQ-ELISA Kit, torpedo/Egfr IQ-ELISA Kit, torpedo/egfr IQ-ELISA Kit, EGFR12 IQ-ELISA Kit, EGFR15 IQ-ELISA Kit, egfr1 IQ-ELISA Kit, Erbb2 IQ-ELISA Kit, ERBB IQ-ELISA Kit, ERBB1 IQ-ELISA Kit, HER1 IQ-ELISA Kit, PIG61 IQ-ELISA Kit, mENA IQ-ELISA Kit, ErbB-1 IQ-ELISA Kit, Errp IQ-ELISA Kit, 9030024J15Rik IQ-ELISA Kit, AI552599 IQ-ELISA Kit, Erbb IQ-ELISA Kit, Errb1 IQ-ELISA Kit, Wa5 IQ-ELISA Kit, wa-2 IQ-ELISA Kit, wa2 IQ-ELISA Kit, epidermal growth factor receptor IQ-ELISA Kit, Epidermal growth factor receptor IQ-ELISA Kit, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) IQ-ELISA Kit, EGFR IQ-ELISA Kit, Egfr IQ-ELISA Kit, egfra IQ-ELISA Kit, egfr1 IQ-ELISA Kit, LOC5564544 IQ-ELISA Kit
    UniProt
    Q6QT04
    Pathways
    NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine
Sie sind hier:
Kundenservice