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ACVRL1 ELISA Kit
ACVRL1
Reaktivität: Maus
Colorimetric
Sandwich ELISA
0.41-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktdetails ACVRL1 ELISA Kit
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Target
Alle ACVRL1 ELISA Kits anzeigen
ACVRL1
(Activin A Receptor Type II-Like 1 (ACVRL1))
Reaktivität
Alle Reaktivitäten für ACVRL1 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
0.41-100 ng/mL
Untere Nachweisgrenze
0.41 ng/mL
Applikation
ELISA
Verwendungszweck
Mouse ALK-1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu ACVRL1
(ausblenden)
Target
Alle ACVRL1 ELISA Kits anzeigen
ACVRL1
(Activin A Receptor Type II-Like 1 (ACVRL1))
Andere Bezeichnung
ALK-1 (ACVRL1 Produkte )
Synonyme
ACVRL1 ELISA Kit, vgb ELISA Kit, alk1 ELISA Kit, zehn1109 ELISA Kit, hm:zehn1109 ELISA Kit, ACVRLK1 ELISA Kit, ALK-1 ELISA Kit, ALK1 ELISA Kit, HHT ELISA Kit, HHT2 ELISA Kit, ORW2 ELISA Kit, SKR3 ELISA Kit, TSR-I ELISA Kit, R-3 ELISA Kit, R3 ELISA Kit, SETHKIR ELISA Kit, AI115505 ELISA Kit, AI427544 ELISA Kit, Acvrlk1 ELISA Kit, Alk1 ELISA Kit, activin A receptor like type 1 ELISA Kit, activin A receptor type II-like 1 ELISA Kit, activin A receptor, type II-like 1 ELISA Kit, ACVRL1 ELISA Kit, acvrl1 ELISA Kit, Acvrl1 ELISA Kit
Gen-ID
20568
UniProt
P97430
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