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CD31 ELISA Kit
PECAM1
Reaktivität: Human
Colorimetric
Sandwich ELISA
15-6000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktdetails CD31 ELISA Kit
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Target
Alle CD31 (PECAM1) ELISA Kits anzeigen
CD31 (PECAM1)
(Platelet/endothelial Cell Adhesion Molecule (PECAM1))
Reaktivität
Alle Reaktivitäten für CD31 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
15-6000 pg/mL
Untere Nachweisgrenze
15 pg/mL
Applikation
ELISA
Verwendungszweck
Human PECAM-1 (CD31) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivität
15 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples10 - 100 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CD31
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Target
Alle CD31 (PECAM1) ELISA Kits anzeigen
CD31 (PECAM1)
(Platelet/endothelial Cell Adhesion Molecule (PECAM1))
Andere Bezeichnung
PECAM-1 (PECAM1 Produkte )
Synonyme
CD31 ELISA Kit, CD31/EndoCAM ELISA Kit, GPIIA' ELISA Kit, PECA1 ELISA Kit, PECAM-1 ELISA Kit, endoCAM ELISA Kit, C85791 ELISA Kit, Cd31 ELISA Kit, Pecam ELISA Kit, platelet/endothelial cell adhesion molecule 1 ELISA Kit, platelet and endothelial cell adhesion molecule 1 ELISA Kit, pecam1 ELISA Kit, PECAM1 ELISA Kit, Pecam1 ELISA Kit
Hintergrund
Gene Names: PECAM1 Protein names: Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)
Gen-ID
5175
UniProt
P16284
Pathways
Regulation of Actin Filament Polymerization
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