NFKBIA ELISA Kit

Produktnummer ABIN6244346

Produktdetails NFKBIA ELISA Kit

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human IkB-alpha/NF-kappa-BIA ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human IkB-alpha.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Andere Bezeichnung: IkB-alpha (NF-kappa-BIA) (NFKBIA Produkte)

Synonyme: IKBA ELISA Kit, MAD-3 ELISA Kit, NFKBI ELISA Kit, AI462015 ELISA Kit, Nfkbi ELISA Kit, RL/IF-1 ELISA Kit, ECI-6/IKBA ELISA Kit, NFKBIA ELISA Kit, ikbaB ELISA Kit, wu:fb81f08 ELISA Kit, zgc:56666 ELISA Kit, zgc:77616 ELISA Kit, ikbaA ELISA Kit, wu:fj16a07 ELISA Kit, zgc:85769 ELISA Kit, NFKB inhibitor alpha ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a ELISA Kit, NFKBIA ELISA Kit, Nfkbia ELISA Kit, nfkbiab ELISA Kit, nfkbiaa ELISA Kit

Gen-ID: 4792

UniProt: P25963

Pathways: NF-kappaB Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling