Reactive Oxygen Species (ROS) Fluorometric Assay Kit

Produktnummer ABIN5708944

Kurzübersicht für Reactive Oxygen Species (ROS) Fluorometric Assay Kit (ABIN5708944)

Target: Reactive Oxygen Species (ROS)

Nachweismethode: Fluorometric

Applikation: Biochemical Assay (BCA)

Proben: Cell Culture Cells, Tissue Samples

Analytische Methode: Quantitative

Produktdetails

Verwendungszweck: This kit can be used for detection of ROS content in cells and tissue samples.

Produktmerkmale: DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

Benötigtes Material: Fluorescence Microplate reader, Fluorescence Spectrophotometry, Flow Cytometry

Anwendungsinformationen

Testdauer: 1 h

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect from light.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Haltbarkeit: 12 months

Antigendetails

Target: Reactive Oxygen Species (ROS)

Andere Bezeichnung: Reactive Oxygen Species