Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

SGK1 ELISA Kit

SGK1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690949
  • Target Alle SGK1 ELISA Kits anzeigen
    SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
    Reaktivität
    • 4
    • 2
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human SGK1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human SGK1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften SGK1 ELISA Kit
    Top Product
    Discover our top product SGK1 ELISA Kit
  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle SGK1 ELISA Kits anzeigen
    SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
    Andere Bezeichnung
    SGK1 (SGK1 Produkte)
    Synonyme
    SGK ELISA Kit, Sgk ELISA Kit, sgk-B ELISA Kit, sgk2 ELISA Kit, cb1083 ELISA Kit, sgk ELISA Kit, wu:fc20a09 ELISA Kit, sgk-A ELISA Kit, sgk1 ELISA Kit, sgk1-a ELISA Kit, sgk1-b ELISA Kit, serum/glucocorticoid regulated kinase 1 ELISA Kit, Serine/threonine-protein kinase sgk-1 ELISA Kit, serum/glucocorticoid regulated kinase 1 S homeolog ELISA Kit, serum/glucocorticoid regulated kinase 1 L homeolog ELISA Kit, SGK1 ELISA Kit, Sgk1 ELISA Kit, sgk-1 ELISA Kit, sgk1.S ELISA Kit, sgk1 ELISA Kit, sgk1.L ELISA Kit
    Gen-ID
    6446
    UniProt
    O00141
    Pathways
    MAPK Signalweg, Notch Signalweg, Steroid Hormone Mediated Signaling Pathway
Kundenservice