PPP1R2 ELISA Kit

Produktnummer ABIN5690938

Produktdetails PPP1R2 ELISA Kit

Target: PPP1R2 (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 2 (PPP1R2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PP1 Inhibitor-2/IPP-2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human PP1 Inhibitor-2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu PPP1R2

Target: PPP1R2 (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 2 (PPP1R2))

Andere Bezeichnung: PP1 Inhibitor-2 (IPP-2 (PPP1R2 Produkte)

Synonyme: DDBDRAFT_0167872 ELISA Kit, DDBDRAFT_0233418 ELISA Kit, DDB_0167872 ELISA Kit, DDB_0233418 ELISA Kit, IPP-2 ELISA Kit, IPP2 ELISA Kit, 0610025N14Rik ELISA Kit, 2310007G06Rik ELISA Kit, 4930440J04Rik ELISA Kit, 5430408E15Rik ELISA Kit, D16Ertd248e ELISA Kit, protein phosphatase inhibitor 2 ELISA Kit, protein phosphatase 1 regulatory inhibitor subunit 2 ELISA Kit, protein phosphatase 1, regulatory (inhibitor) subunit 2 ELISA Kit, dpiA ELISA Kit, Tsp_08270 ELISA Kit, PPP1R2 ELISA Kit, Ppp1r2 ELISA Kit

Gen-ID: 5504

UniProt: P41236

Pathways: PI3K-Akt Signalweg, Cellular Glucan Metabolic Process