TNFRSF1A ELISA Kit
TNFRSF1A
Reaktivität: Hund
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690781
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Target
Alle TNFRSF1A ELISA Kits anzeigen
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TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
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Reaktivität
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Hund
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Canine (dog) TNF RI/TNFRSF1A ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Spezifität
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This ELISA antibody pair recognizes Canine (dog) TNF RI.
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Lagerung
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4 °C
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Haltbarkeit
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6 months
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Target
Alle TNFRSF1A ELISA Kits anzeigen
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TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
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Andere Bezeichnung
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TNF RI (TNFRSF1A (TNFRSF1A Produkte)
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Synonyme
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CD120a ELISA Kit, FPF ELISA Kit, MS5 ELISA Kit, TBP1 ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR1-d2 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, p55 ELISA Kit, p55-R ELISA Kit, p60 ELISA Kit, TNF-R1 ELISA Kit, TNF-alphaR1 ELISA Kit, TNFRI ELISA Kit, TNFRp55 ELISA Kit, TNFalpha-R1 ELISA Kit, Tnfr-2 ELISA Kit, Tnfr1 ELISA Kit, fpf ELISA Kit, tbp1 ELISA Kit, p55-r ELISA Kit, tnf-r ELISA Kit, tnfar ELISA Kit, tnfr1 ELISA Kit, cd120a ELISA Kit, tnfr55 ELISA Kit, tnfr60 ELISA Kit, xtnfr1 ELISA Kit, wu:fj65b10 ELISA Kit, zgc:85914 ELISA Kit, TNFR I ELISA Kit, TNFRSF1A ELISA Kit, TNFA ELISA Kit, cTNF ELISA Kit, TNF receptor superfamily member 1A ELISA Kit, tumor necrosis factor receptor superfamily, member 1a ELISA Kit, TNF receptor superfamily member 1A S homeolog ELISA Kit, tumor necrosis factor ELISA Kit, TNFRSF1A ELISA Kit, Tnfrsf1a ELISA Kit, tnfrsf1a.S ELISA Kit, tnfrsf1a ELISA Kit, TNF ELISA Kit
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NCBI Accession
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XP_854474
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Pathways
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NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway
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