CGRP ELISA Kit

Produktnummer ABIN5690747

Produktdetails CGRP ELISA Kit

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Untere Nachweisgrenze: 0.1 ng/mL

Applikation: ELISA

Verwendungszweck: Human/Mouse/Rat CGRP-I EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat Dynorphin

Sensitivität: 0.2 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu CGRP

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Andere Bezeichnung: CGRP-I (CALCA Produkte)

Synonyme: CALC1 ELISA Kit, CGRP ELISA Kit, CGRP-I ELISA Kit, CGRP1 ELISA Kit, CT ELISA Kit, KC ELISA Kit, CALC ELISA Kit, CALCA ELISA Kit, CALC3 ELISA Kit, ci-ct ELISA Kit, calca ELISA Kit, CA ELISA Kit, CGRP-1 ELISA Kit, Calc ELISA Kit, Calc1 ELISA Kit, Cgrp ELISA Kit, Ct ELISA Kit, Ctn ELISA Kit, calcitonin ELISA Kit, CAL6 ELISA Kit, Cal1 ELISA Kit, RATCAL6 ELISA Kit, CGRPI ELISA Kit, calc ELISA Kit, cgrp ELISA Kit, zgc:92886 ELISA Kit, calcitonin related polypeptide alpha ELISA Kit, calcitonin related polypeptide beta ELISA Kit, calcitonin-related polypeptide alpha ELISA Kit, calcitonin ELISA Kit, calcitonin/calcitonin-related polypeptide, alpha ELISA Kit, calcitonin-related polypeptide beta ELISA Kit, calcitonin gene-related peptide 2 ELISA Kit, CALCA ELISA Kit, CALCB ELISA Kit, ci-ct ELISA Kit, calca ELISA Kit, Calca ELISA Kit, LOC100008771 ELISA Kit

Gen-ID: 24241

UniProt: P01256

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour