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JKAMP ELISA Kit

JKAMP Reaktivität: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Produktnummer ABIN5674944
  • Target Alle JKAMP ELISA Kits anzeigen
    JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.781 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.781 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0.35 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product JKAMP ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle JKAMP ELISA Kits anzeigen
    JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
    Andere Bezeichnung
    JNK1/MAPK8 Associated Membrane Protein (JKAMP) (JKAMP Produkte)
    Synonyme
    C14orf100 ELISA Kit, HSPC213 ELISA Kit, HSPC327 ELISA Kit, JAMP ELISA Kit, 1200003C05Rik ELISA Kit, 2310047L11Rik ELISA Kit, Jamp ELISA Kit, RGD1308917 ELISA Kit, JNK1/MAPK8 associated membrane protein ELISA Kit, JNK1/MAPK8-associated membrane protein ELISA Kit, JKAMP ELISA Kit, Jkamp ELISA Kit
    Hintergrund
    Alternative name: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4
    Gen-ID
    51528
    UniProt
    Q9P055
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