PPP2R4 ELISA Kit

Produktnummer ABIN5674923

Produktdetails PPP2R4 ELISA Kit

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Bindungsspezifität: Regulatory Subunit 4

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0062 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PPP2R4

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Andere Bezeichnung: Protein Phosphatase 2A Activator, (PPP2R4) (PPP2R4 Produkte)

Synonyme: PP2A ELISA Kit, PR53 ELISA Kit, PTPA ELISA Kit, 2610042B21Rik ELISA Kit, C77440 ELISA Kit, N28142 ELISA Kit, PTPRA ELISA Kit, Ppp2r4 ELISA Kit, fc23d07 ELISA Kit, ppp2r4 ELISA Kit, wu:fc23d07 ELISA Kit, zgc:92766 ELISA Kit, pp2a ELISA Kit, ppp2r4.L ELISA Kit, pr53 ELISA Kit, ptpa ELISA Kit, protein phosphatase 2 phosphatase activator ELISA Kit, protein phosphatase 2 protein activator ELISA Kit, protein phosphatase 2 phosphatase activator L homeolog ELISA Kit, PTPA ELISA Kit, Ptpa ELISA Kit, ptpa ELISA Kit, ptpa.L ELISA Kit

Hintergrund: Alternative name: PP2A, PR53, PTPA, Phosphotyrosyl Phosphatase Activator, PP2A Phosphatase Activator, PP2A, subunit B', PR53 isoform, Serine/threonine-protein phosphatase 2A activator

Gen-ID: 110854

UniProt: P58389

Pathways: PI3K-Akt Signalweg, M Phase, Hepatitis C, Toll-Like Receptors Cascades