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SLC9A3 ELISA Kit

SLC9A3 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674915
  • Target Alle SLC9A3 ELISA Kits anzeigen
    SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0093 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle SLC9A3 ELISA Kits anzeigen
    SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))
    Andere Bezeichnung
    Sodium/Hydrogen Exchanger 3 (NHE3) (SLC9A3 Produkte)
    Synonyme
    SLC9A3 ELISA Kit, 9030624O13Rik ELISA Kit, AI930210 ELISA Kit, NHE-3 ELISA Kit, NHE3 ELISA Kit, Nhe3 ELISA Kit, solute carrier family 9 member A3 ELISA Kit, sodium/hydrogen exchanger 3 ELISA Kit, Sodium/hydrogen exchanger 3 ELISA Kit, putative sodium/hydrogen exchanger 3 (nhe3) ELISA Kit, solute carrier family 9 (sodium/hydrogen exchanger), member 3 ELISA Kit, SLC9A3 ELISA Kit, LOC5570922 ELISA Kit, GL50803_102647 ELISA Kit, Smp_045680 ELISA Kit, Slc9a3 ELISA Kit
    Hintergrund
    Alternative name: SLC9A3, Solute Carrier Family 9 Member 3, Sodium-hydrogen antiporter 3, Na(+)/H(+) exchanger 3
    Gen-ID
    6550
    UniProt
    P48764
    Pathways
    Proton Transport
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