PTPN11 ELISA Kit

Produktnummer ABIN5674897

Produktdetails PTPN11 ELISA Kit

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.31 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PTPN11

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Andere Bezeichnung: Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) (PTPN11 Produkte)

Synonyme: BPTP3 ELISA Kit, CFC ELISA Kit, NS1 ELISA Kit, PTP-1D ELISA Kit, PTP2C ELISA Kit, SH-PTP2 ELISA Kit, SH-PTP3 ELISA Kit, SHP2 ELISA Kit, 2700084A17Rik ELISA Kit, AW536184 ELISA Kit, PTP1D ELISA Kit, SAP-2 ELISA Kit, SHP-2 ELISA Kit, Shp2 ELISA Kit, Syp ELISA Kit, SYP ELISA Kit, bptp3 ELISA Kit, cfc ELISA Kit, ns1 ELISA Kit, ptp-2 ELISA Kit, ptp2c ELISA Kit, ptpn11 ELISA Kit, ptpn11-a ELISA Kit, ptpn11-b ELISA Kit, shp-2 ELISA Kit, shp2 ELISA Kit, fa14b09 ELISA Kit, wu:fa14b09 ELISA Kit, wu:fi24f03 ELISA Kit, zgc:55388 ELISA Kit, zgc:63553 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 S homeolog ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, a ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, b ELISA Kit, PTPN11 ELISA Kit, Ptpn11 ELISA Kit, ptpn11.S ELISA Kit, ptpn11a ELISA Kit, ptpn11b ELISA Kit

Substanzklasse: Viral Protein

Hintergrund: Alternative name: BPTP3, CFC, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, Noonan Syndrome 1, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C

Gen-ID: 19247

UniProt: P35235

Pathways: JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effekt