SDHB ELISA Kit

Produktnummer ABIN5674884

Produktdetails SDHB ELISA Kit

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0041 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Andere Bezeichnung: Succinate Dehydrogenase Complex Subunit B (SDHB) (SDHB Produkte)

Synonyme: succinate dehydrogenase 2-3 ELISA Kit, CG3283 ELISA Kit, Dmel\\CG3283 ELISA Kit, Ip ELISA Kit, SDH ELISA Kit, SDH-IP ELISA Kit, SDH-Ip ELISA Kit, SDHb ELISA Kit, sdhB ELISA Kit, CWS2 ELISA Kit, IP ELISA Kit, PGL4 ELISA Kit, SDH1 ELISA Kit, SDH2 ELISA Kit, SDHIP ELISA Kit, 0710008N11Rik ELISA Kit, fj46d06 ELISA Kit, wu:fj46d06 ELISA Kit, succinate dehydrogenase complex iron sulfur subunit B ELISA Kit, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3 ELISA Kit, Succinate dehydrogenase, subunit B (iron-sulfur) ELISA Kit, succinate dehydrogenase complex subunit B, iron sulfur (Ip) ELISA Kit, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) ELISA Kit, succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog ELISA Kit, SDHB ELISA Kit, SDH2-3 ELISA Kit, SdhB ELISA Kit, sdhb ELISA Kit, Sdhb ELISA Kit, sdhb.S ELISA Kit

Hintergrund: Alternative name: SDH1, SDH, Ip, Iron Sulfur, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gen-ID: 298596

UniProt: P21913