PPP3R1 ELISA Kit

Produktnummer ABIN5674873

Produktdetails PPP3R1 ELISA Kit

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Bindungsspezifität: Regulatory Subunit 1

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 15.5 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PPP3R1

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Andere Bezeichnung: Protein Phosphatase 3, (PPP3R1) (PPP3R1 Produkte)

Synonyme: si:ch211-274c22.8 ELISA Kit, canb ELISA Kit, CALNB1 ELISA Kit, CNB ELISA Kit, CNB1 ELISA Kit, CaNB1 ELISA Kit, Cnb1 ELISA Kit, MCIP1 ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha a ELISA Kit, protein phosphatase 3 regulatory subunit B, alpha S homeolog ELISA Kit, protein phosphatase 3 regulatory subunit B, alpha ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) ELISA Kit, ppp3r1a ELISA Kit, ppp3r1.S ELISA Kit, PPP3R1 ELISA Kit, Ppp3r1 ELISA Kit

Hintergrund: Alternative name: CALNB1, CNB, CNB1, Calcineurin B Type I, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

Gen-ID: 5534

UniProt: P63098

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling