ADH7 ELISA Kit

Produktnummer ABIN5674863

Produktdetails ADH7 ELISA Kit

Target: ADH7 (Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0058 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ADH7

Target: ADH7 (Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7))

Andere Bezeichnung: Alcohol Dehydrogenase 7 (ADH7) (ADH7 Produkte)

Synonyme: ADH4 ELISA Kit, AI325182 ELISA Kit, Adh-3 ELISA Kit, Adh-3e ELISA Kit, Adh-3t ELISA Kit, Adh3 ELISA Kit, Adh3-e ELISA Kit, Adh3-t ELISA Kit, Adh4 ELISA Kit, Adt-1 ELISA Kit, ADH7 ELISA Kit, adh6 ELISA Kit, adh7 ELISA Kit, adh7.L ELISA Kit, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ELISA Kit, uncharacterized LOC100232857 ELISA Kit, alcohol dehydrogenase class 4 mu/sigma chain ELISA Kit, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide S homeolog ELISA Kit, ADH7 ELISA Kit, Adh7 ELISA Kit, LOC100232857 ELISA Kit, LOC100512795 ELISA Kit, adh7.S ELISA Kit

Hintergrund: Alternative name: Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase

Gen-ID: 171178

UniProt: P41682