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CYP11B2 ELISA Kit

CYP11B2 Reaktivität: Maus Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5674859
  • Target Alle CYP11B2 ELISA Kits anzeigen
    CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))
    Reaktivität
    • 2
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0117 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle CYP11B2 ELISA Kits anzeigen
    CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))
    Andere Bezeichnung
    Aldosterone Synthase (ALDOS) (CYP11B2 Produkte)
    Synonyme
    ALDOS ELISA Kit, CPN2 ELISA Kit, CYP11B ELISA Kit, CYP11BL ELISA Kit, CYPXIB2 ELISA Kit, P-450C18 ELISA Kit, P450C18 ELISA Kit, P450aldo ELISA Kit, Cpn2 ELISA Kit, Cyp11b ELISA Kit, Cyp11b-2 ELISA Kit, Cp45as ELISA Kit, Cyp11b3 ELISA Kit, RNCP45AS ELISA Kit, cytochrome P450 family 11 subfamily B member 2 ELISA Kit, cytochrome P450, family 11, subfamily b, polypeptide 2 ELISA Kit, aldosterone synthase ELISA Kit, cytochrome P450, family 11, subfamily B, polypeptide 2 ELISA Kit, cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2 ELISA Kit, CYP11B2 ELISA Kit, Cyp11b2 ELISA Kit
    Hintergrund
    Alternative name: CPN2, CYP11B, CYP11BL, CYP11B2, P450C18, P450aldo, Steroid 18-hydroxylase, Aldosterone-synthesizing enzyme, Cytochrome P450 Family 11 Subfamily B Polypeptide 2
    Gen-ID
    11677
    UniProt
    P45376
    Pathways
    ACE Inhibitor Pathway, Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process, Feeding Behaviour
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