CYP2C19 ELISA Kit

Produktnummer ABIN5674819

Produktdetails CYP2C19 ELISA Kit

Target: CYP2C19 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 19 (CYP2C19))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0056 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu CYP2C19

Target: CYP2C19 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 19 (CYP2C19))

Andere Bezeichnung: Cytochrome P450 2C19 (CYP2C19) (CYP2C19 Produkte)

Synonyme: CPCJ ELISA Kit, CYP2C ELISA Kit, P450C2C ELISA Kit, P450IIC19 ELISA Kit, cytochrome P450 family 2 subfamily C member 19 ELISA Kit, cytochrome P450, family 2, subfamily C, polypeptide 19 ELISA Kit, cytochrome P450 2C19 ELISA Kit, CYP2C19 ELISA Kit

Hintergrund: Alternative name: CPCJ, CYP2C, CYPIIC17, CYPIIC19, P450C2C, Cytochrome P450-11A, Cytochrome P450-254C, Mephenytoin 4-hydroxylase, limonene 6-monooxygenase

Gen-ID: 1557

UniProt: P33261