CYP11A1 ELISA Kit

Produktnummer ABIN5674818

Produktdetails CYP11A1 ELISA Kit

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0133 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu CYP11A1

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Andere Bezeichnung: Cytochrome P450 11A1 (CYP11A1) (CYP11A1 Produkte)

Synonyme: CYP11A ELISA Kit, CYP11A1 ELISA Kit, CYPXIA1 ELISA Kit, P450SCC ELISA Kit, Cyp11a ELISA Kit, Cypxia1 ELISA Kit, D9Ertd411e ELISA Kit, P450scc ELISA Kit, Scc ELISA Kit, cscc ELISA Kit, P450(scc) ELISA Kit, SSC ELISA Kit, cytochrome P450 family 11 subfamily A member 1 ELISA Kit, cholesterol side-chain cleavage enzyme, mitochondrial-like ELISA Kit, cytochrome P450 cholesterol side-chain cleavage ELISA Kit, cytochrome P450, family 11, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450, family 11, subfamily A, polypeptide 1 ELISA Kit, CYP11A1 ELISA Kit, LOC101827747 ELISA Kit, Cyp11a1 ELISA Kit

Hintergrund: Alternative name: CYP11A, P450SCC, Cholesterol desmolase, Cholesterol Side Chain Cleavage Enzyme, Cytochrome P450 Family 11 Subfamily A Polypeptide 1

Gen-ID: 13070

UniProt: Q9QZ82

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin