SLC30A5 ELISA Kit

Produktnummer ABIN5674770

Produktdetails SLC30A5 ELISA Kit

Target: SLC30A5 (Solute Carrier Family 30 (Zinc Transporter), Member 5 (SLC30A5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0107 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SLC30A5

Target: SLC30A5 (Solute Carrier Family 30 (Zinc Transporter), Member 5 (SLC30A5))

Andere Bezeichnung: Solute Carrier Family 30, Member 5 (SLC30A5) (SLC30A5 Produkte)

Synonyme: zgc:92436 ELISA Kit, wu:fi03d07 ELISA Kit, SLC30A5 ELISA Kit, slc30a5 ELISA Kit, znt5 ELISA Kit, ztl1 ELISA Kit, znt-5 ELISA Kit, zntl1 ELISA Kit, YUP8H12.8 ELISA Kit, YUP8H12_8 ELISA Kit, zinc transporter 5 precursor ELISA Kit, ZNT5 ELISA Kit, ZNTL1 ELISA Kit, ZTL1 ELISA Kit, ZnT-5 ELISA Kit, 1810010K08Rik ELISA Kit, AF233321 ELISA Kit, Zntl1 ELISA Kit, ZnT5 ELISA Kit, solute carrier family 30 member 5 ELISA Kit, solute carrier family 30 (zinc transporter), member 5 ELISA Kit, solute carrier family 30 (zinc transporter), member 5 L homeolog ELISA Kit, zinc transporter 5 precursor ELISA Kit, Slc30a5 ELISA Kit, slc30a5 ELISA Kit, SLC30A5 ELISA Kit, slc30a5.L ELISA Kit, ZIP5 ELISA Kit

Hintergrund: Alternative name: ZTL1, ZnT-5, ZNT5, ZNTL1, Zinc Transporter 5, ZnT-like transporter 1

Gen-ID: 64924

UniProt: Q8TAD4

Pathways: Peptide Hormone Metabolism, Transition Metal Ion Homeostasis, Proton Transport