SLC2A2 ELISA Kit

Produktnummer ABIN5674769

Produktdetails SLC2A2 ELISA Kit

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.33 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SLC2A2

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Andere Bezeichnung: Glucose Transporter 2 (GLUT2) (SLC2A2 Produkte)

Synonyme: glut2 ELISA Kit, wu:fb62h10 ELISA Kit, slc2a2 ELISA Kit, MGC83262 ELISA Kit, SLC2A2 ELISA Kit, GLUT2 ELISA Kit, GTT2 ELISA Kit, Glut2 ELISA Kit, AI266973 ELISA Kit, Glut-2 ELISA Kit, solute carrier family 2 (facilitated glucose transporter), member 2 ELISA Kit, solute carrier family 2 member 2 L homeolog ELISA Kit, solute carrier family 2 member 2 ELISA Kit, solute carrier family 2, facilitated glucose transporter member 2 ELISA Kit, slc2a2 ELISA Kit, slc2a2.L ELISA Kit, SLC2A2 ELISA Kit, LOC100534484 ELISA Kit, Slc2a2 ELISA Kit

Hintergrund: Alternative name: SLC2A2, SLC2-A2, Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver

UniProt: P12336

Pathways: Warburg Effekt