ATP6AP2 ELISA Kit

Produktnummer ABIN5674751

Produktdetails ATP6AP2 ELISA Kit

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0112 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Abstract: ATP6AP2 Produkte

Synonyme: APT6M8-9 ELISA Kit, ATP6IP2 ELISA Kit, ATP6M8-9 ELISA Kit, ELDF10 ELISA Kit, M8-9 ELISA Kit, MRXE ELISA Kit, MSTP009 ELISA Kit, XMRE ELISA Kit, (P)RR ELISA Kit, 5730403E06Rik ELISA Kit, Atp6ip2 ELISA Kit, tj6 ELISA Kit, wss ELISA Kit, arcl ELISA Kit, j6b7 ELISA Kit, stv1 ELISA Kit, tj6m ELISA Kit, tj6s ELISA Kit, vph1 ELISA Kit, atp6a2 ELISA Kit, atp6n1d ELISA Kit, ATP6AP2 ELISA Kit, DKFZp459G0918 ELISA Kit, atp6ap2 ELISA Kit, wu:fj36c03 ELISA Kit, wu:fq15e04 ELISA Kit, zgc:73194 ELISA Kit, ATPase H+ transporting accessory protein 2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 ELISA Kit, ATPase H+ transporting V0 subunit d2 ELISA Kit, ATPase H+ transporting V0 subunit a2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog ELISA Kit, ATP6AP2 ELISA Kit, Atp6ap2 ELISA Kit, ATP6V0D2 ELISA Kit, atp6v0a2 ELISA Kit, atp6ap2.S ELISA Kit, atp6ap2 ELISA Kit

Hintergrund: Alternative name: ATP6IP2, CAPER, ELDF10, XMRE, Renin/prorenin receptor, Embryonic liver differentiation factor 10, Vacuolar ATP synthase membrane sector-associated protein M8-9

Gen-ID: 10159

UniProt: O75787

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones