ATP2B2 ELISA Kit

Produktnummer ABIN5674663

Produktdetails ATP2B2 ELISA Kit

Target: ATP2B2 (ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0112 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ATP2B2

Target: ATP2B2 (ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2))

Abstract: ATP2B2 Produkte

Synonyme: PMCA2 ELISA Kit, PMCA2a ELISA Kit, PMCA2i ELISA Kit, ATP2B4 ELISA Kit, ATP2B2 ELISA Kit, PMCA ELISA Kit, D6Abb2e ELISA Kit, Tmy ELISA Kit, dfw ELISA Kit, jog ELISA Kit, wms ELISA Kit, wri ELISA Kit, ATPase plasma membrane Ca2+ transporting 2 ELISA Kit, ATPase, Ca++ transporting, plasma membrane 2 ELISA Kit, ATP2B2 ELISA Kit, Atp2b2 ELISA Kit, atp2b2 ELISA Kit

Hintergrund: Alternative name: PMCA2, Plasma Membrane Ca2+ Pump 2, Plasma Membrane Calcium-Transporting ATPase 2

Gen-ID: 491

UniProt: Q01814

Pathways: Sensory Perception of Sound, Regulation of Cell Size, Ribonucleoside Biosynthetic Process