WNT11 ELISA Kit

Produktnummer ABIN5660002

Produktdetails WNT11 ELISA Kit

Target: WNT11 (Wingless-Type MMTV Integration Site Family, Member 11 (WNT11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 11 (WNT11). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 11 (WNT11) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 11 (WNT11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 11 (WNT11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 11 (WNT11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 11 (WNT11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 11 (WNT11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 11 (WNT11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu WNT11

Target: WNT11 (Wingless-Type MMTV Integration Site Family, Member 11 (WNT11))

Andere Bezeichnung: Wingless Type MMTV Integration Site Family, Member 11 (WNT11 Produkte)

Synonyme: HWNT11 ELISA Kit, Wnt11r ELISA Kit, Xwnt11 ELISA Kit, Xwnt-11 ELISA Kit, wnt11-r ELISA Kit, XWnt-11 ELISA Kit, wnt11b ELISA Kit, wnt11b-2 ELISA Kit, Slb/Wnt11 ELISA Kit, cb748 ELISA Kit, slb ELISA Kit, Wnt12 ELISA Kit, WNT11 ELISA Kit, wnt-11 ELISA Kit, xwnt11 ELISA Kit, Wnt family member 11 ELISA Kit, wingless-type MMTV integration site family member 11 L homeolog ELISA Kit, wingless-type MMTV integration site family, member 11B ELISA Kit, wingless-type MMTV integration site family, member 11 ELISA Kit, protein Wnt-11-like ELISA Kit, wingless-type MMTV integration site family, member 10B ELISA Kit, wingless-type MMTV integration site family, member 11B L homeolog ELISA Kit, WNT11 ELISA Kit, Wnt11 ELISA Kit, wnt11.L ELISA Kit, wnt11b ELISA Kit, wnt11 ELISA Kit, LOC100229189 ELISA Kit, Wnt10b ELISA Kit, wnt11b.L ELISA Kit

Hintergrund:

Gene Name: Wingless Type MMTV Integration Site Family, Member 11

Gen-ID: 7481

UniProt: O96014

Pathways: WNT Signalweg, Cell-Cell Junction Organization