Versican CLIA Kit

Produktnummer ABIN5659918

Produktdetails Versican CLIA Kit

Target: Versican (Vcan)

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 312.5 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Versican (VS). No significant cross-reactivity or interference between Versican (VS) and analogues was observed.

Sensitivität: 12.59 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Versican (VS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Versican (VS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Versican (VS) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Versican (Vcan)

Andere Bezeichnung: Versican (Vcan Produkte)

Synonyme: br146 CLIA Kit, cspg2 CLIA Kit, cspg2a CLIA Kit, sb:cb63 CLIA Kit, versican CLIA Kit, CSPG2 CLIA Kit, Cspg2 CLIA Kit, wgn CLIA Kit, ervr CLIA Kit, pg-m CLIA Kit, wgn1 CLIA Kit, Xversican CLIA Kit, dcan CLIA Kit, cspg2b CLIA Kit, fb99b07 CLIA Kit, wu:fb99b07 CLIA Kit, ERVR CLIA Kit, GHAP CLIA Kit, PG-M CLIA Kit, WGN CLIA Kit, WGN1 CLIA Kit, 5430420N07Rik CLIA Kit, 9430051N09 CLIA Kit, DPEAAE CLIA Kit, NG2 CLIA Kit, PG-M(V0) CLIA Kit, PG-M(V1) CLIA Kit, hdf CLIA Kit, versican a CLIA Kit, versican CLIA Kit, versican b CLIA Kit, vcana CLIA Kit, VCAN CLIA Kit, Vcan CLIA Kit, vcan CLIA Kit, vcanb CLIA Kit

Hintergrund:

Gene Name: Versican

Gene Aliases: CSPG2, VCAN, ERVR, PG-M, WGN1, Chondroitin Sulfate Proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding, Large fibroblast proteoglycan

Gen-ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process