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ERBB3 ELISA Kit

ERBB3 Reaktivität: Maus Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Produktnummer ABIN5659802
  • Target Alle ERBB3 ELISA Kits anzeigen
    ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
    Reaktivität
    • 10
    • 3
    • 2
    • 2
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    7.81 pg/mL - 500 pg/mL
    Untere Nachweisgrenze
    7.81 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). No significant cross-reactivity or interference between V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) and analogues was observed.
    Sensitivität
    3.4 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ERBB3 ELISA Kits anzeigen
    ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
    Andere Bezeichnung
    V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ERBB3 Produkte)
    Synonyme
    ErbB-3 ELISA Kit, HER3 ELISA Kit, LCCS2 ELISA Kit, MDA-BF-1 ELISA Kit, c-erbB-3 ELISA Kit, c-erbB3 ELISA Kit, erbB3-S ELISA Kit, p180-ErbB3 ELISA Kit, p45-sErbB3 ELISA Kit, p85-sErbB3 ELISA Kit, ERBB3 ELISA Kit, C76256 ELISA Kit, Erbb-3 ELISA Kit, Erbb3r ELISA Kit, Her3 ELISA Kit, nuc-ErbB3 ELISA Kit, erb-b2 receptor tyrosine kinase 3 ELISA Kit, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) ELISA Kit, ERBB3 ELISA Kit, Erbb3 ELISA Kit
    Hintergrund

    Gene Name: V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3

    Gene Aliases: HER3, MDA-BF-1, C-ErbB3, LCCS2, ErbB3-S, P180-ErbB3, P45-SErbB3, P85-SErbB3, Receptor Tyrosine-Protein Kinase ErbB-3, Lethal Congenital Contracture Syndrome 2

    Gen-ID
    13867
    UniProt
    Q61526
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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