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UBA52 ELISA Kit

UBA52 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Produktnummer ABIN5659724
  • Target Alle UBA52 ELISA Kits anzeigen
    UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 (UBA52))
    Reaktivität
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). No significant cross-reactivity or interference between Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) and analogues was observed.
    Sensitivität
    0.116 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle UBA52 ELISA Kits anzeigen
    UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 (UBA52))
    Andere Bezeichnung
    Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52 Produkte)
    Synonyme
    CEP52 ELISA Kit, HUBCEP52 ELISA Kit, L40 ELISA Kit, RPL40 ELISA Kit, D8Ertd21e ELISA Kit, Gm1863 ELISA Kit, Rps27a ELISA Kit, Ubb ELISA Kit, Ubc ELISA Kit, UBCEP2 ELISA Kit, UB-RPL40 ELISA Kit, MGC80109 ELISA Kit, cep52 ELISA Kit, hubcep52 ELISA Kit, rpl40 ELISA Kit, RPS27A ELISA Kit, UBB ELISA Kit, UBC ELISA Kit, wu:fa91f08 ELISA Kit, zgc:123344 ELISA Kit, UBA52 ELISA Kit, ubiquitin A-52 residue ribosomal protein fusion product 1 ELISA Kit, ubiquitin A-52 residue ribosomal protein fusion product 1 L homeolog ELISA Kit, UBA52 ELISA Kit, Uba52 ELISA Kit, uba52.L ELISA Kit, uba52 ELISA Kit
    Hintergrund

    Gene Name: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1

    Gene Aliases: CEP52, HUBCEP52, RPL40, 60S Ribosomal Protein L40, Ubiquitin Carboxyl Extension Protein 52

    Gen-ID
    7311
    UniProt
    P62987
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation
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