Tyrosine Hydroxylase ELISA Kit

Produktnummer ABIN5659712

Produktdetails Tyrosine Hydroxylase ELISA Kit

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH). No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.

Sensitivität: 0.062 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine Hydroxylase (TH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine Hydroxylase (TH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine Hydroxylase (TH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine Hydroxylase (TH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase (TH Produkte)

Synonyme: DYT14 ELISA Kit, DYT5b ELISA Kit, TYH ELISA Kit, The ELISA Kit, CG10118 ELISA Kit, DH65B ELISA Kit, DTH ELISA Kit, Dmel\\CG10118 ELISA Kit, Pale ELISA Kit, Ple ELISA Kit, TH ELISA Kit, Th ELISA Kit, VII ELISA Kit, dTH1 ELISA Kit, dTH65B ELISA Kit, pale/ple ELISA Kit, th ELISA Kit, dyt14 ELISA Kit, dyt5b ELISA Kit, tyh ELISA Kit, tyrosine hydroxylase ELISA Kit, pale ELISA Kit, tyrosine 3-monooxygenase ELISA Kit, tyrosine hydroxylase S homeolog ELISA Kit, TH ELISA Kit, Th ELISA Kit, ple ELISA Kit, th ELISA Kit, th.S ELISA Kit, LOC100008895 ELISA Kit

Hintergrund:

Gene Name: Tyrosine Hydroxylase

Gene Aliases: TYH, DYT5b, Tyrosine 3-Monooxygenase, Dystonia 14

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour