TSPO ELISA Kit

Produktnummer ABIN5659497

Produktdetails TSPO ELISA Kit

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Translocator Protein (TSPO). No significant cross-reactivity or interference between Translocator Protein (TSPO) and analogues was observed.

Sensitivität: 0.113 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocator Protein (TSPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocator Protein (TSPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocator Protein (TSPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocator Protein (TSPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocator Protein (TSPO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocator Protein (TSPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Andere Bezeichnung: Translocator Protein (TSPO Produkte)

Synonyme: LOC732875 ELISA Kit, LOC100136652 ELISA Kit, LOC100280949 ELISA Kit, BZRP ELISA Kit, TSPO ELISA Kit, Bzrp ELISA Kit, IBP ELISA Kit, PBR ELISA Kit, Tspo1 ELISA Kit, MBR ELISA Kit, PTBZR02 ELISA Kit, Ptbzr ELISA Kit, RATPTBZR02 ELISA Kit, BPBS ELISA Kit, DBI ELISA Kit, PBS ELISA Kit, PKBS ELISA Kit, PTBR ELISA Kit, mDRC ELISA Kit, pk18 ELISA Kit, peripheral-type benzodiazepine receptor ELISA Kit, Peripheral-type benzodiazepine receptor ELISA Kit, putative peripheral-type benzodiazepine receptor ELISA Kit, translocator protein ELISA Kit, translocator protein homolog ELISA Kit, hypothetical protein ELISA Kit, LOC732875 ELISA Kit, MAV_3896 ELISA Kit, xccb100_2086 ELISA Kit, Smp_102510 ELISA Kit, Sros_1911 ELISA Kit, tspo ELISA Kit, LOC100280949 ELISA Kit, PGTG_16395 ELISA Kit, TSPO ELISA Kit, Tspo ELISA Kit

Hintergrund:

Gene Name: Translocator Protein

Gene Aliases: IBP, PKBS, PBR, pk18, mDRC, MBR, BZRP, DBI, PTBR, Mitochondrial benzodiazepine receptor, Peripheral-Type Benzodiazepine Receptor/Recognition Site

Gen-ID: 706

UniProt: P30536

Pathways: Cellular Response to Molecule of Bacterial Origin