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TGFBR1 ELISA Kit

TGFBR1 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5659461
  • Target Alle TGFBR1 ELISA Kits anzeigen
    TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))
    Reaktivität
    • 4
    • 2
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta Receptor I (TGFbR1). No significant cross-reactivity or interference between Transforming Growth Factor Beta Receptor I (TGFbR1) and analogues was observed.
    Sensitivität
    0.117 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Receptor I (TGFbR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta Receptor I (TGFbR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta Receptor I (TGFbR1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta Receptor I (TGFbR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle TGFBR1 ELISA Kits anzeigen
    TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))
    Andere Bezeichnung
    Transforming Growth Factor Beta Receptor I (TGFBR1 Produkte)
    Synonyme
    AAT5 ELISA Kit, ACVRLK4 ELISA Kit, ALK-5 ELISA Kit, ALK5 ELISA Kit, LDS1A ELISA Kit, LDS2A ELISA Kit, MSSE ELISA Kit, SKR4 ELISA Kit, TGFR-1 ELISA Kit, aat5 ELISA Kit, acvrlk4 ELISA Kit, alk-5 ELISA Kit, alk5 ELISA Kit, lds1a ELISA Kit, lds2a ELISA Kit, skr4 ELISA Kit, tgfr-1 ELISA Kit, TGFBR1 ELISA Kit, x-trr1 ELISA Kit, AU017191 ELISA Kit, Alk-5 ELISA Kit, TbetaR-I ELISA Kit, TbetaRI ELISA Kit, Alk5 ELISA Kit, Skr4 ELISA Kit, Tgfr-1 ELISA Kit, tbetaR-I ELISA Kit, tgfbr1 ELISA Kit, zgc:123263 ELISA Kit, transforming growth factor beta receptor 1 ELISA Kit, transforming growth factor beta receptor I ELISA Kit, transforming growth factor beta receptor I S homeolog ELISA Kit, transforming growth factor, beta receptor I ELISA Kit, transforming growth factor, beta receptor 1 ELISA Kit, transforming growth factor, beta receptor 1 a ELISA Kit, TGFBR1 ELISA Kit, tgfbr1 ELISA Kit, tgfbr1.S ELISA Kit, Tgfbr1 ELISA Kit, tgfbr1a ELISA Kit
    Hintergrund

    Gene Name: Transforming Growth Factor Beta Receptor I

    Gene Aliases: ACVRLK4, AAT5, ALK5, SKR4, TGFR-1, Activin A Receptor Type II-Like Kinase 53 kDa, Activin receptor-like kinase 5, Serine/threonine-protein kinase receptor R4

    Gen-ID
    7046
    UniProt
    P36897
    Pathways
    Growth Factor Binding
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