TDGF1 CLIA Kit

Produktnummer ABIN5659119

Produktdetails TDGF1 CLIA Kit

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Teratocarcinoma Derived Growth Factor 1 (TDGF1). No significant cross-reactivity or interference between Teratocarcinoma Derived Growth Factor 1 (TDGF1) and analogues was observed.

Sensitivität: 5.32 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Teratocarcinoma Derived Growth Factor 1 (TDGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Teratocarcinoma Derived Growth Factor 1 (TDGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Teratocarcinoma Derived Growth Factor 1 (TDGF1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Teratocarcinoma Derived Growth Factor 1 (TDGF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Teratocarcinoma Derived Growth Factor 1 (TDGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Andere Bezeichnung: Teratocarcinoma Derived Growth Factor 1 (TDGF1 Produkte)

Synonyme: CR CLIA Kit, CRGF CLIA Kit, CRIPTO CLIA Kit, CR1 CLIA Kit, cripto CLIA Kit, cb85 CLIA Kit, tdgf1 CLIA Kit, wu:fe01d04 CLIA Kit, zgc:109829 CLIA Kit, Cripto-1 CLIA Kit, cr1 CLIA Kit, cr1a CLIA Kit, frl-1 CLIA Kit, frl1 CLIA Kit, xcr1 CLIA Kit, TDGF1 CLIA Kit, tdgf3 CLIA Kit, cripto-3 CLIA Kit, teratocarcinoma-derived growth factor 1 CLIA Kit, teratocarcinoma-derived growth factor 1, member 3 L homeolog CLIA Kit, teratocarcinoma-derived growth factor 1, member 1 CLIA Kit, TDGF1 CLIA Kit, Tdgf1 CLIA Kit, tdgf1 CLIA Kit, tdgf1.3.L CLIA Kit, tdgf1.1 CLIA Kit

Hintergrund:

Gene Name: Teratocarcinoma Derived Growth Factor 1

Gene Aliases: CR, CRGF, CRIPTO, Cripto-1, Epidermal growth factor-like cripto protein CR1, Cripto-1 growth factor

Pathways: EGFR Signaling Pathway