SULF1 CLIA Kit

Produktnummer ABIN5658989

Produktdetails SULF1 CLIA Kit

Target: SULF1 (Sulfatase 1 (SULF1))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25 pg/mL - 400 pg/mL

Untere Nachweisgrenze: 6.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Sulfatase 1 (SULF1). No significant cross-reactivity or interference between Sulfatase 1 (SULF1) and analogues was observed.

Sensitivität: 0.23 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Sulfatase 1 (SULF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Sulfatase 1 (SULF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Sulfatase 1 (SULF1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfatase 1 (SULF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfatase 1 (SULF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: SULF1 (Sulfatase 1 (SULF1))

Andere Bezeichnung: Sulfatase 1 (SULF1 Produkte)

Synonyme: HSULF-1 CLIA Kit, SULF-1 CLIA Kit, CG6725 CLIA Kit, DSulf CLIA Kit, DSulf1 CLIA Kit, Dmel\\CG6725 CLIA Kit, Dsulf1 CLIA Kit, Neu5 CLIA Kit, sulf1 CLIA Kit, AI467640 CLIA Kit, AW121680 CLIA Kit, mKIAA1077 CLIA Kit, fk14e08 CLIA Kit, wu:fk14e08 CLIA Kit, SULF1 CLIA Kit, sulf-1 CLIA Kit, XtSulf1 CLIA Kit, hsulf-1 CLIA Kit, XtSulf-1 CLIA Kit, sulfatase 1 CLIA Kit, Sulfated CLIA Kit, extracellular sulfatase SULF-1 homolog CLIA Kit, SULF1 CLIA Kit, Sulf1 CLIA Kit, sulf1 CLIA Kit, LOC410233 CLIA Kit

Hintergrund:

Gene Name: Sulfatase 1

Gene Aliases: HSULF-1, Extracellular sulfatase Sulf-1

Gen-ID: 23213

UniProt: Q8IWU6

Pathways: EGFR Signaling Pathway