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SLC39A6 ELISA Kit

SLC39A6 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Produktnummer ABIN5658873
  • Target Alle SLC39A6 ELISA Kits anzeigen
    SLC39A6 (Solute Carrier Family 39 (Zinc Transporter), Member 6 (SLC39A6))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 39, Member 6 (SLC39A6). No significant cross-reactivity or interference between Solute Carrier Family 39, Member 6 (SLC39A6) and analogues was observed.
    Sensitivität
    0.114 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 39, Member 6 (SLC39A6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 39, Member 6 (SLC39A6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 39, Member 6 (SLC39A6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 39, Member 6 (SLC39A6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 39, Member 6 (SLC39A6) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 39, Member 6 (SLC39A6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle SLC39A6 ELISA Kits anzeigen
    SLC39A6 (Solute Carrier Family 39 (Zinc Transporter), Member 6 (SLC39A6))
    Andere Bezeichnung
    Solute Carrier Family 39, Member 6 (SLC39A6 Produkte)
    Synonyme
    liv1 ELISA Kit, wu:fc25a09 ELISA Kit, ZIP6 ELISA Kit, Ermelin ELISA Kit, LIV-1 ELISA Kit, solute carrier family 39 (zinc transporter), member 6 ELISA Kit, solute carrier family 39 member 6 ELISA Kit, solute carrier family 39 (metal ion transporter), member 6 ELISA Kit, slc39a6 ELISA Kit, SLC39A6 ELISA Kit, Slc39a6 ELISA Kit
    Hintergrund

    Gene Name: Solute Carrier Family 39, Member 6

    Gene Aliases: LIV-1, Zinc Transporter Protein 6, Estrogen-regulated protein LIV-1, Zrt- and Irt-like protein 6, Zinc transporter ZIP6

    Gen-ID
    25800
    UniProt
    Q13433
    Pathways
    Transition Metal Ion Homeostasis
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