SLC30A8 ELISA Kit

Produktnummer ABIN5658864

Produktdetails SLC30A8 ELISA Kit

Target: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 30 Member 8 (SLC30A8). No significant cross-reactivity or interference between Solute Carrier Family 30 Member 8 (SLC30A8) and analogues was observed.

Sensitivität: 0.057 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30 Member 8 (SLC30A8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30 Member 8 (SLC30A8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30 Member 8 (SLC30A8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 30 Member 8 (SLC30A8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30 Member 8 (SLC30A8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30 Member 8 (SLC30A8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SLC30A8

Target: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Andere Bezeichnung: Solute Carrier Family 30 Member 8 (SLC30A8 Produkte)

Synonyme: C820002P14Rik ELISA Kit, ZnT-8 ELISA Kit, ZnT8 ELISA Kit, slc30a3 ELISA Kit, znt8 ELISA Kit, ZNT8 ELISA Kit, E130106K10Rik ELISA Kit, Gm212 ELISA Kit, wu:fe49g03 ELISA Kit, RGD1305098 ELISA Kit, solute carrier family 30 member 8 ELISA Kit, solute carrier family 30 (zinc transporter), member 8 ELISA Kit, solute carrier family 30 (zinc transporter), member 8 L homeolog ELISA Kit, solute carrier family 30, member 10 ELISA Kit, SLC30A8 ELISA Kit, Slc30a8 ELISA Kit, slc30a8.L ELISA Kit, slc30a8 ELISA Kit, Slc30a10 ELISA Kit

Hintergrund:

Gene Name: Solute Carrier Family 30 Member 8

Gene Aliases: ZNT8, Zinc Transporter 8

Gen-ID: 169026

UniProt: Q8IWU4

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis