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SLC5A2 ELISA Kit

SLC5A2 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5658852
  • Target Alle SLC5A2 ELISA Kits anzeigen
    SLC5A2 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 2 (SLC5A2))
    Reaktivität
    • 3
    • 2
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.781 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.781 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Sodium/Glucose Cotransporter 2 (SGLT2). No significant cross-reactivity or interference between Sodium/Glucose Cotransporter 2 (SGLT2) and analogues was observed.
    Sensitivität
    0.28 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Glucose Cotransporter 2 (SGLT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Glucose Cotransporter 2 (SGLT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sodium/Glucose Cotransporter 2 (SGLT2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sodium/Glucose Cotransporter 2 (SGLT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle SLC5A2 ELISA Kits anzeigen
    SLC5A2 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 2 (SLC5A2))
    Andere Bezeichnung
    Sodium/Glucose Cotransporter 2 (SLC5A2 Produkte)
    Synonyme
    SLC5A2 ELISA Kit, slc5a2 ELISA Kit, DKFZp469E0433 ELISA Kit, zgc:85792 ELISA Kit, MGC83377 ELISA Kit, SGLT2 ELISA Kit, Sglt2 ELISA Kit, solute carrier family 5 member 2 ELISA Kit, solute carrier family 5 (sodium/glucose cotransporter), member 2 ELISA Kit, solute carrier family 5 (sodium/glucose cotransporter), member 2 L homeolog ELISA Kit, SLC5A2 ELISA Kit, slc5a2 ELISA Kit, slc5a2.L ELISA Kit, Slc5a2 ELISA Kit
    Hintergrund

    Gene Name: Sodium/Glucose Cotransporter 2

    Gene Aliases: SLC5A2, Solute Carrier Family 5 Member 2, Low affinity sodium-glucose cotransporter, Na(+)/glucose cotransporter 2

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