STAT1 ELISA Kit

Produktnummer ABIN5658794

Produktdetails STAT1 ELISA Kit

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 1 (STAT1). No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 1 (STAT1) and analogues was observed.

Sensitivität: 0.115 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 1 (STAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 1 (STAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Andere Bezeichnung: Signal Transducer And Activator Of Transcription 1 (STAT1 Produkte)

Synonyme: CANDF7 ELISA Kit, ISGF-3 ELISA Kit, STAT91 ELISA Kit, 2010005J02Rik ELISA Kit, AA408197 ELISA Kit, XStat1 ELISA Kit, STAT4 ELISA Kit, DD6G4-4 ELISA Kit, STAT ELISA Kit, STAT1 ELISA Kit, dZ182H3.3 ELISA Kit, si:by134g18.2 ELISA Kit, si:by51f19.1 ELISA Kit, si:dz182h3.3 ELISA Kit, si:dz199m19.1 ELISA Kit, si:dz73o4.2 ELISA Kit, stat1 ELISA Kit, signal transducer and activator of transcription 1 ELISA Kit, signal transducer and activator of transcription 1 L homeolog ELISA Kit, signal transducer and activator of transcription 1, 91kDa ELISA Kit, signal transducer and activator of transcription 4 ELISA Kit, signal transducer and activator of transcription 1a ELISA Kit, STAT1 ELISA Kit, Stat1 ELISA Kit, stat1.L ELISA Kit, stat4 ELISA Kit, stat1a ELISA Kit

Hintergrund:

Gene Name: Signal Transducer And Activator Of Transcription 1

Gene Aliases: ISGF-3, STAT91, Transcription Factor ISGF-3 Components p91/p84

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events