RCBTB2 ELISA Kit

Produktnummer ABIN5658360

Produktdetails RCBTB2 ELISA Kit

Target: RCBTB2 (Regulator of Chromosome Condensation (RCC1) and BTB (POZ) Domain Containing Protein 2 (RCBTB2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of RCC1 And BTB Domain Containing Protein 2 (RCBTB2). No significant cross-reactivity or interference between RCC1 And BTB Domain Containing Protein 2 (RCBTB2) and analogues was observed.

Sensitivität: 0.124 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RCC1 And BTB Domain Containing Protein 2 (RCBTB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RCC1 And BTB Domain Containing Protein 2 (RCBTB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RCC1 And BTB Domain Containing Protein 2 (RCBTB2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RCC1 And BTB Domain Containing Protein 2 (RCBTB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RCBTB2

Target: RCBTB2 (Regulator of Chromosome Condensation (RCC1) and BTB (POZ) Domain Containing Protein 2 (RCBTB2))

Andere Bezeichnung: RCC1 And BTB Domain Containing Protein 2 (RCBTB2 Produkte)

Synonyme: chc1l ELISA Kit, chc1l-a ELISA Kit, MGC53589 ELISA Kit, CHC1L ELISA Kit, 2610028E02Rik ELISA Kit, 2810420M18Rik ELISA Kit, AI448345 ELISA Kit, AW240694 ELISA Kit, Chc1l ELISA Kit, RC/BTB2 ELISA Kit, regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2 S homeolog ELISA Kit, RCC1 and BTB domain containing protein 2 ELISA Kit, regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2 ELISA Kit, rcbtb2.S ELISA Kit, RCBTB2 ELISA Kit, Rcbtb2 ELISA Kit

Hintergrund:

Gene Name: RCC1 And BTB Domain Containing Protein 2

Gene Aliases: CHC1L, RLG, RCC1-like G exchanging factor, Regulator Of Chromosome Condensation And BTB(POZ)Domain Containing Protein 2, Chromosome Condensation 1-Like

Gen-ID: 1102

UniProt: O95199