PTGS2 ELISA Kit

Produktnummer ABIN5658148

Produktdetails PTGS2 ELISA Kit

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2). No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.

Sensitivität: 0.129 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PTGS2

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Andere Bezeichnung: Prostaglandin Endoperoxide Synthase 2 (PTGS2 Produkte)

Synonyme: COX-2 ELISA Kit, COX2 ELISA Kit, GRIPGHS ELISA Kit, PGG/HS ELISA Kit, PGHS-2 ELISA Kit, PHS-2 ELISA Kit, hCox-2 ELISA Kit, Cox-2 ELISA Kit, Pghs2 ELISA Kit, TIS10 ELISA Kit, Cox2 ELISA Kit, cox-2 ELISA Kit, CEF147 ELISA Kit, PGHS2 ELISA Kit, PHSII ELISA Kit, cox2 ELISA Kit, gripghs ELISA Kit, pgg/hs ELISA Kit, pghs-2 ELISA Kit, phs-2 ELISA Kit, PTGS2 ELISA Kit, hcox-2 ELISA Kit, fj02a10 ELISA Kit, ptgs2 ELISA Kit, unp1239 ELISA Kit, wu:fj02a10 ELISA Kit, zCOX-2 ELISA Kit, si:dkey-97o5.6 ELISA Kit, prostaglandin-endoperoxide synthase 2 ELISA Kit, prostaglandin G/H synthase 2 ELISA Kit, prostaglandin-endoperoxide synthase 2S homeolog ELISA Kit, prostaglandin-endoperoxide synthase 2a ELISA Kit, prostaglandin-endoperoxide synthase 2b ELISA Kit, PTGS2 ELISA Kit, Ptgs2 ELISA Kit, LOC100136456 ELISA Kit, ptgs2.S ELISA Kit, ptgs2 ELISA Kit, ptgs2a ELISA Kit, ptgs2b ELISA Kit

Hintergrund:

Gene Name: Prostaglandin Endoperoxide Synthase 2

Gene Aliases: PGG/HS, PGHS2, PHS2, HCox2, COX2, Cyclooxygenase 2, Prostaglandin G/H Synthase And Cyclooxygenase, Prostaglandin H2 synthase 2

Gen-ID: 5743

UniProt: P35354

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation