PARP1 CLIA Kit

Produktnummer ABIN5657973

Produktdetails PARP1 CLIA Kit

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Untere Nachweisgrenze: 3.12 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.

Sensitivität: 0.11 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Poly ADP Ribose Polymerase (PARP) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: Poly ADP Ribose Polymerase (PARP1 Produkte)

Synonyme: ADPRT CLIA Kit, ADPRT 1 CLIA Kit, ADPRT1 CLIA Kit, ARTD1 CLIA Kit, PARP CLIA Kit, PARP-1 CLIA Kit, PPOL CLIA Kit, pADPRT-1 CLIA Kit, Adprt CLIA Kit, Parp-1 CLIA Kit, 5830444G22Rik CLIA Kit, AI893648 CLIA Kit, Adprp CLIA Kit, Adprt1 CLIA Kit, C80510 CLIA Kit, parp-1 CLIA Kit, sPARP-1 CLIA Kit, BEST:LD21673 CLIA Kit, CG17685 CLIA Kit, CG17696 CLIA Kit, CG17718 CLIA Kit, CG40411 CLIA Kit, D.PARP CLIA Kit, Dm.pARTa CLIA Kit, Dmel\\CG40411 CLIA Kit, LD21673.3prime CLIA Kit, PARP1 CLIA Kit, dPARP CLIA Kit, parp CLIA Kit, adprt1 CLIA Kit, padprt-1 CLIA Kit, ppol CLIA Kit, si:dkey-206f10.3 CLIA Kit, wu:fc60f12 CLIA Kit, zgc:110092 CLIA Kit, poly(ADP-ribose) polymerase 1 CLIA Kit, poly (ADP-ribose) polymerase 1 CLIA Kit, poly (ADP-ribose) polymerase family, member 1 CLIA Kit, Poly-(ADP-ribose) polymerase CLIA Kit, poly(ADP-ribose) polymerase 1 L homeolog CLIA Kit, PARP1 CLIA Kit, Parp1 CLIA Kit, Parp CLIA Kit, parp1.L CLIA Kit, parp1 CLIA Kit

Hintergrund:

Gene Name: Poly ADP Ribose Polymerase

Gene Aliases: PARP1, ADPRT, ADPRT1, PPOL, pADPRT-1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1

Gen-ID: 142

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location