PDGFRA ELISA Kit

Produktnummer ABIN5657904

Produktdetails PDGFRA ELISA Kit

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Receptor Alpha (PDGFRa). No significant cross-reactivity or interference between Platelet Derived Growth Factor Receptor Alpha (PDGFRa) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Receptor Alpha (PDGFRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Receptor Alpha (PDGFRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Receptor Alpha (PDGFRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Receptor Alpha (PDGFRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Alpha (PDGFRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Alpha (PDGFRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: Platelet Derived Growth Factor Receptor Alpha (PDGFRA Produkte)

Synonyme: AI115593 ELISA Kit, CD140a ELISA Kit, Pdgfr-2 ELISA Kit, APDGFR ELISA Kit, PDGFACE ELISA Kit, CD140A ELISA Kit, PDGFR-2 ELISA Kit, PDGFR2 ELISA Kit, RHEPDGFRA ELISA Kit, PDGFR ELISA Kit, PDGFR-A ELISA Kit, platelet derived growth factor receptor alpha ELISA Kit, platelet derived growth factor receptor, alpha polypeptide ELISA Kit, platelet-derived growth factor receptor, alpha polypeptide L homeolog ELISA Kit, platelet-derived growth factor receptor, alpha polypeptide ELISA Kit, PDGFRA ELISA Kit, Pdgfra ELISA Kit, pdgfra.L ELISA Kit

Hintergrund:

Gene Name: Platelet Derived Growth Factor Receptor Alpha

Gene Aliases: CD140a, PDGFR2, Rhe-PDGFRA, CD140 antigen-like family member A, Platelet-derived growth factor receptor 2, Alpha-type platelet-derived growth factor receptor

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling