Myoglobin CLIA Kit

Produktnummer ABIN5657073

Produktdetails Myoglobin CLIA Kit

Target: Myoglobin (MB)

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Myoglobin (MYO). No significant cross-reactivity or interference between Myoglobin (MYO) and analogues was observed.

Sensitivität: 2.14 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Myoglobin (MYO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myoglobin (MYO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myoglobin (MYO) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myoglobin (MYO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myoglobin (MYO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Myoglobin (MB)

Andere Bezeichnung: Myoglobin (MB Produkte)

Synonyme: PVALB CLIA Kit, AI325109 CLIA Kit, zgc:65819 CLIA Kit, zgc:77764 CLIA Kit, MB CLIA Kit, DKFZp468H096 CLIA Kit, myg CLIA Kit, mb CLIA Kit, MYF4 CLIA Kit, bHLHc3 CLIA Kit, myo CLIA Kit, Myoglobin CLIA Kit, myoglobin CLIA Kit, myogenin CLIA Kit, MB CLIA Kit, Mb CLIA Kit, mb CLIA Kit, myg CLIA Kit, Myog CLIA Kit

Hintergrund:

Gene Name: Myoglobin

Gene Aliases: PVALB, MB

Gen-ID: 17189

UniProt: P04247

Pathways: Brown Fat Cell Differentiation