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CCL2 CLIA Kit

CCL2 Reaktivität: Kaninchen Chemiluminescent Sandwich ELISA 625.00 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5656943
  • Target Alle CCL2 CLIA Kits anzeigen
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    Reaktivität
    • 12
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Kaninchen
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    625.00 pg/mL - 40000 pg/mL
    Untere Nachweisgrenze
    625.00 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 1 (MCP1). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 1 (MCP1) and analogues was observed.
    Sensitivität
    21.5 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 - 3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monocyte Chemotactic Protein 1 (MCP1) level in the sample or standard.,
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CCL2 CLIA Kits anzeigen
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    Andere Bezeichnung
    Monocyte Chemotactic Protein 1 (CCL2 Produkte)
    Synonyme
    GDCF-2 CLIA Kit, HC11 CLIA Kit, HSMCR30 CLIA Kit, MCAF CLIA Kit, MCP-1 CLIA Kit, MCP1 CLIA Kit, SCYA2 CLIA Kit, SMC-CF CLIA Kit, AI323594 CLIA Kit, JE CLIA Kit, Scya2 CLIA Kit, Sigje CLIA Kit, MCP-1A CLIA Kit, MCP1A CLIA Kit, C-C motif chemokine ligand 2 CLIA Kit, chemokine (C-C motif) ligand 2 CLIA Kit, C-C motif chemokine 2 CLIA Kit, CCL2 CLIA Kit, Ccl2 CLIA Kit, LOC101120093 CLIA Kit
    Hintergrund

    Gene Name: Monocyte Chemotactic Protein 1

    Gene Aliases: CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE

    Gen-ID
    100009130
    UniProt
    P28292
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion
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