NR1H3 ELISA Kit

Produktnummer ABIN5656475

Produktdetails NR1H3 ELISA Kit

Target: NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Liver X Receptor Alpha (LXRa). No significant cross-reactivity or interference between Liver X Receptor Alpha (LXRa) and analogues was observed.

Sensitivität: 0.103 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver X Receptor Alpha (LXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver X Receptor Alpha (LXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver X Receptor Alpha (LXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver X Receptor Alpha (LXRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NR1H3

Target: NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))

Andere Bezeichnung: Liver X Receptor Alpha (NR1H3 Produkte)

Synonyme: NR1H3 ELISA Kit, LXR-a ELISA Kit, LXRA ELISA Kit, RLD-1 ELISA Kit, AU018371 ELISA Kit, LXR ELISA Kit, RLD1 ELISA Kit, Unr1 ELISA Kit, LXRalpha ELISA Kit, LXR-alpha ELISA Kit, im:7141239 ELISA Kit, wu:fd59b01 ELISA Kit, zgc:113339 ELISA Kit, zgc:158542 ELISA Kit, nuclear receptor subfamily 1 group H member 3 ELISA Kit, nuclear receptor subfamily 1, group H, member 3 ELISA Kit, NR1H3 ELISA Kit, nr1h3 ELISA Kit, Nr1h3 ELISA Kit

Hintergrund:

Gene Name: Liver X Receptor Alpha

Gene Aliases: NR1-H3, LXR-A, LXRA, RLD-1, Nuclear Receptor Subfamily 1,Group H,Member 3, Oxysterols receptor LXR-alpha

Gen-ID: 22259

UniProt: Q9Z0Y9

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C