LTBP1 ELISA Kit

Produktnummer ABIN5656325

Produktdetails LTBP1 ELISA Kit

Target: LTBP1 (Latent Transforming Growth Factor beta Binding Protein 1 (LTBP1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). No significant cross-reactivity or interference between Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) and analogues was observed.

Sensitivität: 0.053 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu LTBP1

Target: LTBP1 (Latent Transforming Growth Factor beta Binding Protein 1 (LTBP1))

Andere Bezeichnung: Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1 Produkte)

Synonyme: ltgf-1 ELISA Kit, LTBP1 ELISA Kit, 9430031G15Rik ELISA Kit, 9830146M04 ELISA Kit, Ltbp-1 ELISA Kit, Tgfb ELISA Kit, b2b1000Clo ELISA Kit, latent transforming growth factor beta binding protein 1 S homeolog ELISA Kit, latent transforming growth factor beta binding protein 1 ELISA Kit, latent transforming growth factor beta binding protein 2 ELISA Kit, latent TGFbeta binding protein ELISA Kit, latent-transforming growth factor beta-binding protein 1 ELISA Kit, ltbp1.S ELISA Kit, ltbp1 ELISA Kit, LTBP1 ELISA Kit, LTBP2 ELISA Kit, LOC100542113 ELISA Kit, Ltbp1 ELISA Kit

Hintergrund:

Gene Name: Latent Transforming Growth Factor Beta Binding Protein 1

Gene Aliases: TGF-beta1-BP-1, Transforming growth factor beta-1-binding protein 1

Gen-ID: 268977

UniProt: Q8CG19

Pathways: Maintenance of Protein Location, Growth Factor Binding