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Klotho CLIA Kit

KL Reaktivität: Human Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Plasma, Serum
Produktnummer ABIN5656242
  • Target Alle Klotho (KL) CLIA Kits anzeigen
    Klotho (KL)
    Reaktivität
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156.25 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    156.25 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Klotho (KL). No significant cross-reactivity or interference between Klotho (KL) and analogues was observed.
    Sensitivität
    5.08 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 - 3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Klotho (KL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Klotho (KL) level in the sample or standard.,
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Klotho (KL) CLIA Kits anzeigen
    Klotho (KL)
    Andere Bezeichnung
    Klotho (KL Produkte)
    Synonyme
    alpha-kl CLIA Kit, KL CLIA Kit, LOC100223456 CLIA Kit, klotho CLIA Kit, Klotho CLIA Kit, KL CLIA Kit, Kl CLIA Kit, kl CLIA Kit, LOC100533542 CLIA Kit
    Hintergrund

    Gene Name: Klotho

    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Activity, Growth Factor Binding
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